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Genetic Polymorphism In Individuals With Type II Diabetes Mellitus Using PCR-RAPD In Sidoarjo District

Journal of Health Sciences

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Title Genetic Polymorphism In Individuals With Type II Diabetes Mellitus Using PCR-RAPD In Sidoarjo District
 
Creator Mushlih, Miftahul
Kumala Sari , Fitri
A. Hadie, Djauharoh
Ardiansyah, Syahrul
 
Subject Mellitus, Polymorphisms, Metabolic disorder, PCR- RAPD
 
Description Diabetes Mellitus type II (DMT2) is a metabolic disorder which its number increases from year to year. The Control of DMT2 sufferer’s number is difficult because DMT2 is caused by genetic and environmental factors. DMT2 can worsen infectious diseases for people with other infectious diseases. This study aimed to determine the polymorphism of sufferers and non-sufferers of T2DM using PCR-RAPD method. The sample of this study consisted of 60 samples which 30 positive samples and 30 negative samples taken from several clinics in Sidoarjo district. The primer used is A 18 (5'- AGGTGACCGT-3 '). Data analysis was performed using the SPSS 16.00 program and analyzed with a Cross-sectional Chi-square with a 95% confidence level. The results of this study produced 17 bands with length of 197 bp, 239 bp, 269 bp, 319 bp, 390 bp, 530 bp, 588 bp, 686 bp, 777 bp, 972 bp, 1175 bp, 1676 bp, 2780 bp, 3843 bp, 6563 bp, 11072 bp & 18434 bp. Four of them were monomorphic and two bands showed significantly different results at 319 bp (p: 0.035) and 18434 bp (p: 0.004).there were significant differences in the resulting band at 319 (p: 0.035) bp and 18434 bp (p: 0.004). Further analysis needs to be done to confirm the genes involved.
 
Publisher Universitas Nahdlatul Ulama Surabaya
 
Date 2021-05-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/JHS/article/view/1866
10.33086/jhs.v14i02.1866
 
Source Journal of Health Sciences; Vol. 14 No. 02 (2021): Jurnal Ilmiah Kesehatan (Journal of Health Sciences); 153-159
Jurnal Ilmiah Kesehatan; Vol 14 No 02 (2021): Jurnal Ilmiah Kesehatan (Journal of Health Sciences); 153-159
2477-3948
1978-6743
10.33086/jhs.v14i02
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/JHS/article/view/1866/1384
 
Rights Copyright (c) 2021 Miftahul Mushlih Mushlih
https://creativecommons.org/licenses/by-sa/4.0