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Kesesuaian Pemeriksaan jamur antara pewarnaan periodic acid schiff(PAS) dan KOH pada flour albus ibu hamil di RSUD dr Soetomo Surabaya

Journal of Health Sciences

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Title Kesesuaian Pemeriksaan jamur antara pewarnaan periodic acid schiff(PAS) dan KOH pada flour albus ibu hamil di RSUD dr Soetomo Surabaya
 
Creator arya, Shinta
kawilarang, Arthur pohan
 
Subject Flour albus, Kehamilan, Periodic Acid Schiff (PAS), KOH.
 
Description Background: Candidiasis vaginalis is a form of vaginal infection by Candida spp, estimated to occur in women of childbearing age in 70-75% of the cases. Clinical history, symptoms and laboratory examinations are very important to diagnose candidiasis vaginalis. Vaginal discharge examination can be done by direct or wet, and culture examination. Microscopic examination can be used to prove the existence of yeast form of Candida. This method is simple, can be applied in clinical microbiology laboratory with limited resources and is considered effective because it is cheap and results are obtained in a short time compared to culture. Detection of fungi by Periodic Acid Schiff (PAS) staining is visually better than KOH. The aim of this study is to analyze the concordance between Periodic Acid Schiff (PAS) staining and KOH method for fungal detection in flour albus from pregnant women.
Method: This research is a descriptive observational study to figure out the suitability of Periodic Acid Schiff (PAS) staining and KOH method for fungal detection in flour albus from pregnant women with cross sectional approach. Samples were collected in the form of 30 vaginal discharge specimens taken from pregnant women and sent to the Clinical Microbiology Laboratory Dr. Soetomo Surabaya. The study was conducted in July - August 2019.
Result: There was a significant difference in fungal detection between Periodic Acid Schiff (PAS) staining (56.7%, 17 samples) and 20% KOH method (6 samples) out of 30 samples (p=0.017). The concordance between Periodic Acid Schiff (PAS) staining and KOH method for fungal detection in flour albus from pregnant women is low (Kappa=0.321).
Conclusion: Periodic Acid Schiff (PAS) staining detects more fungi when compared to KOH, and therefore is considered as a better method.
Keywords: Flour albus, pregnancy, Periodic Acid Schiff (PAS), KOH.
 
Publisher Universitas Nahdlatul Ulama Surabaya
 
Date 2020-02-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/JHS/article/view/1342
10.33086/jhs.v13i01.1342
 
Source Journal of Health Sciences; Vol. 13 No. 01 (2020): Jurnal Ilmiah Kesehatan (Journal of Health Science); 34-38
Jurnal Ilmiah Kesehatan; Vol 13 No 01 (2020): Jurnal Ilmiah Kesehatan (Journal of Health Science); 34-38
2477-3948
1978-6743
10.33086/jhs.v13i01
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/JHS/article/view/1342/1004
 
Rights Copyright (c) 2020 Shinta arya; Arthur pohan kawilarang
https://creativecommons.org/licenses/by-sa/4.0