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The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture

Indonesian Journal of Medical Laboratory Science and Technology

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Title The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture
 
Creator Turista, Dora Dayu Rahma
Puspitasari, Eka
Razvi, Fanny Kurnanda
 
Subject AB Blood Type
Blood Agar Plates
Defibrination
EDTA
Staphylococcus aureus
 
Description Blood Agar Plates (BAP) are composed of blood as one of the compositions. Sheep’s blood is usually used, but since it is difficult to be obtained, human AB blood type was used as an alternative. In preparing BAP, blood is defibrinated to lyse the blood clotting factors. Blood clots can also be prevented by adding anticoagulants, such as ethylenediaminetetraacetic acid (EDTA). This study aims to investigate the potential use of EDTA as a substitute for defibrination in preparing BAP with human AB blood type. This study employed a completely randomized design with true experimental method using Staphylococcus aureus as the sample. The parameters were the number of colonies, types of hemolysis, and hemolysis zone. The results showed that the S. aureus grown on BAP with EDTA-human AB blood type was 64 colonies (mean), produced β-hemolytic pattern, and 6 mm hemolytic zone. In contrast, the S. aureus grown on BAP with defibrinated human AB blood type showed 82 colonies (mean), β-hemolytic pattern, and 5 mm hemolytic zone. There were significant differences in the number of colonies (0.000 < α) and hemolytic zones (0.02 < α). However, there was no difference in the hemolysis type (both treatments produced β-hemolysis). EDTA was possible to be used as a substitute for defibrination in preparing BAP to assess the hemolysis type of S. aureus, but it might not be able to be used as a benchmark for counting the number of colonies and determining the hemolysis zone of S. aureus.
 
Publisher UNUSA PRESS
 
Date 2021-04-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1923
10.33086/ijmlst.v3i1.1923
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 3 No. 1 (2021): Laboratory innovation : The challenge for medical laboratory ; 64-71
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ; 64-71
2656-9825
2684-6748
10.33086/ijmlst.v3i1
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1923/1339
 
Rights Copyright (c) 2021 Dora Dayu Rahma Turista, Eka Puspitasari, Fanny Kurnanda Razvi
https://creativecommons.org/licenses/by-sa/4.0