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Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli

Indonesian Journal of Medical Laboratory Science and Technology

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Title Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli
 
Creator Prayekti, Endah
Suliati, Suliati
Wulandari, Dwi Agustin
 
Subject Coconut Water
Escherichia coli
Mac Conkey
 
Description Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.
 
Publisher UNUSA PRESS
 
Date 2021-04-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1906
10.33086/ijmlst.v3i1.1906
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 3 No. 1 (2021): Laboratory innovation : The challenge for medical laboratory ; 19-25
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ; 19-25
2656-9825
2684-6748
10.33086/ijmlst.v3i1
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1906/1335
 
Rights Copyright (c) 2021 Endah Prayekti, Suliati, Dwi Agustin Wulandari
https://creativecommons.org/licenses/by-sa/4.0