Record Details

Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument

Indonesian Journal of Medical Laboratory Science and Technology

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Field Value
 
Title Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument
 
Creator Hidayat, Muhammad Taufiq
Prasetyo, Endry Nugroho
 
Subject Basic Laboratory Instrument
PureLink® RNA Mini Kit
RNA Extraction
Staphylococcus aureus
 
Description RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.
 
Publisher UNUSA PRESS
 
Date 2021-10-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1863
10.33086/ijmlst.v3i2.1863
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 3 No. 2 (2021): Molecule analysis to advance laboratory diagnosis; 73-80
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis; 73-80
2656-9825
2684-6748
10.33086/ijmlst.v3i2
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1863/1499
 
Rights Copyright (c) 2021 Muhammad Taufiq Hidayat, Endry Nugroho Prasetyo
https://creativecommons.org/licenses/by-sa/4.0