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PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING

Indonesian Journal of Medical Laboratory Science and Technology

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Title PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING
 
Creator Nunki, Nastasya
Titik Mutiarawati, Diah
Prayekti, Endah
 
Subject Ipomoea batatas L.
Gentian violet
bacteria Gram taining
Bacillus sp.
 
Description Crystal violet and Safranin are dyes in Gram staining, which are carcinogenic. Alternative safe materials are needed to minimize the use of carcinogenic properties. Purple sweet potato (Ipomoea batatas L.) peels were the candidate of the alternative dye source because of its high anthocyanin pigment. The purpose of this study was to determine purple sweet potato (Ipomoea batatas L.) peels extract as an alternative to Gentian violet in Gram staining of bacteria. Extracts obtained from purple sweet potato peels studied with varying concentrations of 50%, 60%, and 75% for 1, 3, and 5 min as a substitute for Gentian violet on Bacillus sp. The parameters observed from this study based on visual field clarity, glass slide cleanliness, contrast, bacterial shape, bacterial colour. Each extract concentration compared with a control group using Gentian violet. The results showed that optimum staining in 50% concentration for 5 min, 60% concentration for 5 min, 75% concentration for 3 min, and 5 min respectively. The present study exhibited the potency of Ipomoea batatas L. peels extract as an alternative staining agent.
 
Publisher UNUSA PRESS
 
Date 2020-08-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1655
10.33086/ijmlst.v2i2.1655
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 2 No. 2 (2020): Increasing Research in Diagnostic Laboratory Testing; 76-84
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing; 76-84
2656-9825
2684-6748
10.33086/ijmlst.v2i2
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1655/1139
 
Rights Copyright (c) 2020 Nastasya Nunki, Diah Titik Mutiarawati, Endah Prayekti
https://creativecommons.org/licenses/by-sa/4.0