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DETERMINATION OF IMMUNOSTIMULANT DOSE RANGE ANDTHE BEST SOAKING TIME DURATIONTO THE PHENOLIC CRUDE EXTRACT BEFOREAeromonas sp. CHALLENGE TEST USING LC50

Samakia : Jurnal Ilmu Perikanan

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Title DETERMINATION OF IMMUNOSTIMULANT DOSE RANGE ANDTHE BEST SOAKING TIME DURATIONTO THE PHENOLIC CRUDE EXTRACT BEFOREAeromonas sp. CHALLENGE TEST USING LC50
PENENTUAN RANGE DOSIS IMUNOSTIMULAN DAN LAMA WAKTUPERENDAMAN TERBAIK PADA EKSTRAK KASAR FENOLGracilaria sp. SEBELUM UJI TANTANG BAKTERI Aeromonas sp. DENGAN MENGGUNAKAN LC50
 
Creator Kurniawati, Ismaningdyah
Maftuch, Maftuch
Hariati, Anik Martinah
 
Subject Gracilaria sp., LC50, maceration techniques.
Gracilariasp., LC50, maserasi.
 
Description Immunostimulatory dose given to the fish to be precise, should not be less and should not be excessive. This is because the immunostimulatory properties which can only work at the optimal dose.Therefore,themethod fordetermining the dosage and administration techniques immunostimulatory should be studied in depth.Research on the determination of the dose and the best maceration time on the crude extract phenol Gracilaria sp. using LC50 is intended to determine how best dose and how long it's best used in the immersion process to extract.rangedose used is 1 g/l; 1,5 g/l; 2 g/l; 3 g/l; 4 g/l and 5 g/l.The results of these observations indicate that doses ≥ 3 g/l has a 10-hour LC50.While the dose ≤ 3 g/l has a 14-hour LC50.However, further research is continued in the time range for prolonged submersion namely 10, 12 and 14 hours.The results showed the soaking time 12 hours showed LC50 15 hours.It concluded that the dose that can be used to test immunostimulant is 1, 1.5 and 3 g/l with a soaking time of less than 12 hours.
Dosisimunostimulan yang diberikan kepada ikan harus tepat, tidak boleh kurang dan tidak boleh berlebihan. Hal ini karena sifat imunostimulan yang hanya dapat bekerja pada dosis yang optimal. Oleh karena itu, metode untuk menentukan dosis dan teknik pemberian imunostimulan harus dikaji secara mendalam. Penelitian tentang penentuandosisdanwaktumaserasiterbaikpadaekstrakkasarfenolGracilaria sp. menggunakan LC50inibertujuanuntukmengetahuiberapadosisterbaikdanberapa lama waktuterbaik yang digunakanpada proses perendamandenganekstrak. Range dosis yang digunakanyaitu 1 g/l; 1,5g/l; 2 gr/l; 3 g/l; 4 g/l dan 5 g/l. Hasilpengamataninimenunjukkanbahwadosis ≥ 3 gr/l memiliki LC50 10 jam. Sedangkandosis ≤ 3 gr/l memiliki LC5014 jam.Penelitianlanjutandilanjutkanpada range waktuuntuk lama perendamanyaitu 10, 12 dan 14 jam. Hasilpenelitianmenunjukkanpada lama perendaman 12 jam menunjukkan LC50 15 jam. Sehinggadapatdisimpulkanbahwadosis yang dapatdigunakanuntukujiimunostimulanadalah 1, 1,5dan 3 g/l dengan lama perendamankurangdari
 
Publisher Faculty of Science and Technology University Ibrahimy
 
Date 2017-04-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://journal.ibrahimy.ac.id/index.php/JSAPI/article/view/266
10.5281/jsapi.v8i1.266
 
Source Samakia : Jurnal Ilmu Perikanan; Vol. 8 No. 1 (2017): Samakia: Jurnal Ilmu Perikanan; 01-05
Samakia : Jurnal Ilmu Perikanan; Vol 8 No 1 (2017): Samakia: Jurnal Ilmu Perikanan; 01-05
2503-2283
2086-3861
10.5281/jsapi.v8i1
 
Language ind
 
Relation https://journal.ibrahimy.ac.id/index.php/JSAPI/article/view/266/252
 
Rights Copyright (c) 2017 Ismaningdyah Kurniawati, Maftuch Maftuch, Anik Martinah Hariati
https://creativecommons.org/licenses/by-nc/4.0