Record Details

UTILIZATION OF 1% OF METHYLENE BLUE IN STAINING HISTOPATHOLOGICAL PREPARATIONS AT ANATOMIC PATHOLOGY LABORATORY

Indonesian Journal of Medical Laboratory Science and Technology

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Field Value
 
Title UTILIZATION OF 1% OF METHYLENE BLUE IN STAINING HISTOPATHOLOGICAL PREPARATIONS AT ANATOMIC PATHOLOGY LABORATORY
 
Creator Rahmawati, Tri
Apriyadi, Yadi
Mamay
 
Subject Cancer
hematoxylin
methylene blue
 
Description Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the staining preparations results as good as those using hematoxylin.
 
Publisher UNUSA PRESS
 
Date 2020-08-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1563
10.33086/ijmlst.v2i2.1563
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 2 No. 2 (2020): Increasing Research in Diagnostic Laboratory Testing; 93-100
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing; 93-100
2656-9825
2684-6748
10.33086/ijmlst.v2i2
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1563/1142
 
Rights Copyright (c) 2020 Tri Rahmawati, Yadi Apriyadi, Mamay
https://creativecommons.org/licenses/by-sa/4.0