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Cytotoxic Activity of Ethanol Extract in Namnam Leaves (cynometra cauliflora l.) to Hela Cell

Walisongo Journal of Chemistry

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Title Cytotoxic Activity of Ethanol Extract in Namnam Leaves (cynometra cauliflora l.) to Hela Cell
 
Creator Anliza, Syarah
Rachmawati, Nurmeily
 
Subject —
Cytotoxic activity; ethanol extract of Namnam leaves; HeLa cell
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Description Cervical cancer is one of the most prominent death cases after breast cancer for a woman in Indonesia. Several treatments can be conducted to cure cancer such as chemotherapy, radiotherapy, tumor surgery, and others. In addition, people can consume some medicines or natural substances that can suppress cancer growth. The natural substances contain active compounds that have the potential as anticancer. One of the natural substances used in this research is the Namnam leaves which grow in Southeast Asia.  The purpose of this research was to determine the potential compounds in Namnam leaves extract by cytotoxic activity testing by using HeLa cells. The active compound in the Namnam leaves extract was obtained by using the maceration method with ethanol for 3x24 hours. The extract was then tested by BSLT method and proliferation by using HeLa cancer cells (ATCC CCL-2). The toxicity results showed that LC50 value was 125,89 μg/mL. This result indicated that the extract belongs to the moderate toxic category and has potential as an anticancer agent. Proliferation test in inhibiting of HeLa cancer cells used Microculture Tetrazolium Technique (MTT) method. The result showed that the extract with a concentration of 25 μg/mL could inhibit the proliferation of HeLa cancer cells by 57,51%.
 
Publisher Department of Chemistry Faculty of Science and Technology Walisongo
 
Contributor —
 
Date 2021-12-15
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
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Format application/pdf
 
Identifier https://journal.walisongo.ac.id/index.php/wjc/article/view/7999
10.21580/wjc.v4i2.7999
 
Source Walisongo Journal of Chemistry; Vol 4, No 2 (2021): Walisongo Journal of Chemistry; 107-112
2621-5985
2549-385X
 
Language eng
 
Relation https://journal.walisongo.ac.id/index.php/wjc/article/view/7999/3690
 
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http://creativecommons.org/licenses/by-nc-sa/4.0