Record Details

CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT

Indonesian Journal of Medical Laboratory Science and Technology

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Title CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT
 
Creator Andini, Ary
Prayekti, Endah
Dyah Wulandari, Devyana
Nidianti, Ersalina
 
Subject Collagen
chitosan
BSLT
wound dressing
ultraviolet
 
Description Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the  best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing  sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into  K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained  collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
 
Publisher UNUSA PRESS
 
Date 2020-04-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1467
10.33086/ijmlst.v2i1.1467
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 2 No. 1 (2020): Laboratory Examinations Support in Medical Toxicology; 21-26
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology; 21-26
2656-9825
2684-6748
10.33086/ijmlst.v2i1
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1467/1051
 
Rights Copyright (c) 2020 Ary Andini, Endah Prayekti, Devyana Dyah Wulandari, Ersalina Nidianti