Record Details

LABORATORY TRIAL OF PROTEIN DETERMINATION IN URINE USING DIFFERENT PH VALUES OF ACETIC ACID AND ACETATE BUFFER METHOD

Indonesian Journal of Medical Laboratory Science and Technology

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Title LABORATORY TRIAL OF PROTEIN DETERMINATION IN URINE USING DIFFERENT PH VALUES OF ACETIC ACID AND ACETATE BUFFER METHOD
 
Creator Rahayu, Dinar
Rustiana, Tuti
 
Subject Proteinuria
acetic acid
acetate buffer
urine pH
buffer
 
Description Abstract
The determination of protein in urine is important in clinical examination along with other parameters in urine. The presence of protein in urine can be interpreted that there is a disorder in kidney. Acid and heat coagulations method is still widely used in many areas to determine protein in urine. In this method, the characteristic of protein that will precipitate in the presence of acid or if exposed to heat is deployed to gain information about the amount of protein. The greater amount of protein, the more prominence is the coagulation. Urine pH also varies according to the condition, classic acidosis will give an acidic urine and the presence of ammonium producing bacteria can cause basic urine. In this research acetic acid method with 6% of CH3COOH and pH value of 2,9 and buffer acetic with pH 4,5 are used to determine the certain amount of protein (+3 value, corresponds with 2-4 mg/dL protein in urine) in varied pH values of urine samples. To compare the results, first in control urine with pH 6,8 the results of both methods is compared and shows no significant different, then the Kruskall-Wallis test is used to compare the results in other pH values to control and the test is shown also there are no significant difference. This shows that either acetic acid at pH 2,9 or acetic buffer at pH 4,5 can be used to determine protein amount in urine.
 
Publisher UNUSA PRESS
 
Date 2020-04-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1459
10.33086/ijmlst.v2i1.1459
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 2 No. 1 (2020): Laboratory Examinations Support in Medical Toxicology; 34-41
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology; 34-41
2656-9825
2684-6748
10.33086/ijmlst.v2i1
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1459/1052
 
Rights Copyright (c) 2020 Dinar Rahayu, Tuti Rustiana