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IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY

Indonesian Journal of Medical Laboratory Science and Technology

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Title IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY
 
Creator Riscahyani, Nosy Maria
Ekawati, Evy Ratnasari
Ngibad, Khoirul
 
Subject Ascorbic acid
papaya
iodimetry
UV-Vis spectrophotometry
 
Description Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.
 
Publisher UNUSA PRESS
 
Date 2019-10-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1291
10.33086/ijmlst.v1i2.1291
 
Source Indonesian Journal of Medical Laboratory Science and Technology; Vol. 1 No. 2 (2019): Laboratory Analysis Applied to Medical Properties; 58-64
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI; Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties; 58-64
2656-9825
2684-6748
10.33086/ijmlst.v1i2
 
Language eng
 
Relation https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1291/928
 
Rights Copyright (c) 2019 Nosy Maria Riscahyani, Evy Ratnasari Ekawati, Khoirul Ngibad
https://creativecommons.org/licenses/by-sa/4.0