Analisis kadar total flavonoid pada daun dan biji pepaya (carica papaya l.) Menggunakan metode spektrofotometer Uv-Vis
Jurnal Ilmiah Farmasi Attamru (JIFA)
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Title |
Analisis kadar total flavonoid pada daun dan biji pepaya (carica papaya l.) Menggunakan metode spektrofotometer Uv-Vis
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Creator |
Asmoro Bangun, Putro Panji
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Description |
Analysis of total flavonoid content of papaya leaf and seed extract (Carica papaya L.) by UV-Vis spectrophotometry method. The purpose of this study was because the utilization of papaya leaves and seeds (Carica papaya L.) was still lacking, and to determine the total flavonoid content contained in papaya leaf and seed extract (Carica papaya L.). Extraction of chemical content from the leaves and seeds of papaya (Carica papaya L.) was carried out by maceration method using 96% ethanol. To determine the levels of flavonoid compounds in the sample extract, a compound analysis was performed using UV-Vis spectrophotometry. From the results of the study obtained a qualitative analysis of the flavonoid compounds of each papaya leaf and seed (Carica papaya L.) from 96% ethanol extract changes to brick red color which indicates a positive flavonoid content. Meanwhile, the absorbance measurement was carried out using UV-Vis spectrophotometry with a wavelength of 510 nm. 5 ppm absorbance value (0.214), 10 ppm absorbance value (0.3799), 15 ppm absorbance value (0.5126), and 20 ppm absorbance value (0.7033). As well as measurement of total flavonoid content of papaya leaves (Carica papaya L.) from 96% ethanol extract of 17.4633 mg QE/g or 1.7463% and total flavonoid content of papaya seeds (Carica papaya L.) from 96% ethanol extract of ,8181 mg QE/g or 1,5818%. These results indicate that papaya leaf extract (Carica papaya L.) has higher flavonoid content than papaya seed extract (Carica papaya L.). Analisis kadar flavonoid total ekstrak daun dan biji pepaya (Carica papaya L.) dengan metode spektrofotometri UV-Vis. Tujuan penelitian ini dilakukan karena pemanfaatan daun dan biji pepaya pepaya (Carica papaya L.) masih kurang, serta untuk mengetahui kadar flavonoid total yang terkandung dalam ekstrak daun dan biji pepaya (Carica papaya L.). Ekstraksi kandungan kimia dari daun dan biji pepaya (Carica papaya L.) dilakukan dengan metode maserasi menggunakan etanol 96%. Untuk menentukan kadar senyawa flavonoid pada ekstrak sampel, maka dilakukan analisis senyawa menggunakan spektrofotometri UV-Vis. Dari hasil penelitian diperoleh analisis kualitatif senyawa flavonoid masing-masing daun dan biji pepaya (Carica papaya L.) dari ekstrak etanol 96% terjadi perubahan menjadi warna merah bata yang menunjukkan positif mengandung flavonoid. Sedangkan Pengukuran absorbansi dilakukan menggunakan spektrofotometri UV-Vis dengan panjang gelombang 510 nm. 5 ppm nilai absorbansinya (0,214), 10 ppm nilai absorbansinya (0,3799), 15 ppm nilai absorbansinya (0,5126), dan 20 ppm nilai absorbansinya (0,7033). Serta pengukuran kadar flavonoid total daun pepaya (Carica papaya L.) dari ekstrak etanol 96% sebesar 17,4633 mg QE/g atau 1,7463 % dan kadar flavonoid total biji pepaya (Carica papaya L.) dari ekstrak etanol 96% sebesar 15,8181 mg QE/g atau 1,5818 %. Hasil tersebut menunjukkan bahwa ekstrak daun pepaya (Carica papaya L.) memiliki kadar flavonoid lebih tinggi dibandingkan dengan ekstrak biji pepaya (Carica papaya L.). |
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Publisher |
Universitas Islam Madura
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Date |
2021-11-19
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
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Format |
application/pdf
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Identifier |
https://journal.uim.ac.id/index.php/Attamru/article/view/1263
10.31102/attamru.v2i1.1263 |
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Source |
Jurnal Ilmiah Farmasi Attamru (JIFA); Vol 2 No 1 (2021): JURNAL ILMIAH FARMASI ATTAMRU (FEBRUARI); 1-5
2723-5629 10.31102/attamru.v2i1 |
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Language |
ind
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Relation |
https://journal.uim.ac.id/index.php/Attamru/article/view/1263/757
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Rights |
Copyright (c) 2021 Jurnal Ilmiah Farmasi Attamru
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