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Efek Penambahan Fraksi Polar F29-F32 Ekstak Metanolik Meniran (Phyllanthus niruri L.) terhadap Daya Hambat Chloramphenicol dan Amoxicillin pada Staphylococcus aureus dan Escherichia coli

Jurnal Bio Komplementer Medicine

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Title Efek Penambahan Fraksi Polar F29-F32 Ekstak Metanolik Meniran (Phyllanthus niruri L.) terhadap Daya Hambat Chloramphenicol dan Amoxicillin pada Staphylococcus aureus dan Escherichia coli
 
Creator Yasmin, Farah
Risandiansyah, Rio
Aini, Noer
 
Description ABSTRACTIntroduction: One solution to overcome antibiotic resistance is addition of adjuvants from herbs. Previous study found the extract of stonebreaker (Phyllanthus niruri, L.) works synergistically with Amoxicillin against E.coli. However, the active compound that interacts with these antibiotics are not yet known. This study separates active compounds with polar fraction and  examines the combination of stonebreaker with antibiotics in inhibiting the development of S. aureus and E. coli.Method: Fractionation was carried out on methanolic simplisia extract of Phyllanthun niruri L. using silica resin with an eluent of 100% methanol. The fractionation results were tested by Thin Layer Chromatography (TLC) using Dragendroff, FeCl3, and Formaldehyde reagents. Single (fraction) and combination (fraction and antibiotic) inhibition was measured using Kirby-Bauer method, and the interaction analysis based on AZDAST (Ameri-Ziaei Double Antibiotic Synergism Test). Statistical tests were used Mann-Whitney with was significance of P<0.05.Result Fractination produced 4 fractions (F29-F32). The results of fractions 29-32 were found to contain phenol compounds, but other staining reagents did not found positive result. Fractions 29, 31 and 32 in stonebreaker had a single inhibitory activity against S. aureus. Fractions 30 and 32 in stonebreaker had a single inhibitory activity against E. coli. The combination of fraction 29 and Amoxicillin (10.6±1.1 mm) had a smaller ZOI than a single fraction ZOI (13.6 ± 1.5 mm). The combination of fraction 30 and Cholamphenicol (17.3±0.57 mm) had a smaller ZOI than a single ZOI Chloramphenicol (19.6±0.57 mm).Conclusion fractions 29-32 were found to contain phenol compounds. Combination of fraction 29 and Amoxicillin are antagonistic to S. aureus and combination of fraction 30 and Chloramphenicol are antagonistic to E.coli. (p<0.05)Keywords: Phyllanthus niruri, L., Polar Fraction, Staphylococcus aureus, Escherichia coli
 
Publisher Jurnal Bio Komplementer Medicine
 
Contributor —
 
Date 2020-03-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://riset.unisma.ac.id/index.php/jbm/article/view/6625
 
Source Jurnal Bio Komplementer Medicine; Vol 7, No 1 (2020)
 
Language eng
 
Relation http://riset.unisma.ac.id/index.php/jbm/article/view/6625/5372
 
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