Pengaruh Air Kelapa (Cocos nucifera L) dengan Medium VW terhadap Pertumbuhan Protocorm Anggrek secara in vitro
Jurnal SAINS ALAMI (Known Nature)
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Title |
Pengaruh Air Kelapa (Cocos nucifera L) dengan Medium VW terhadap Pertumbuhan Protocorm Anggrek secara in vitro
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Creator |
Santoso, Edi
Rahayu, Tintrim Hayati, Ari |
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Description |
Phalaenopsis sp is one of the types of orchids native to Indonesia with high commercial value, but these orchids are quite difficult to cultivate because the seeds are microscopic and do not have endosperm that need to be cultivated in vitro. In vitro orchid cultivation requires a suitable supporting medium. This study aims to determine the effect of coconut water (Cocos nucifera L) using VW medium on the growth of Phalaenopsis sp protocorm orchid in vitro. Research has been carried out at the DD Orchid Nursery Network Culture Laboratory Dadaprejo Junrejo, Batu, East Java, starting from November to January 2020. This study uses a Completely Randomized Design (CRD) to compare different concentrations of coconut water (Cocos nucifera L) as ie, 0%, 15%, 30%, and 60% treatment. Each concentration was repeated 5 times and each repetition consisted of 5 bottles of Phalaenopsis sp. Protocorm and cultured for 4 weeks. The results showed that the highest number of protochromes and shoots were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average number of 64 protocorms and 14 shoots. Keywords: Coconut water (Cocos nucifera L), Phalaenopsis sp., In vitro, growthABSTRAKPhalaenopsis sp merupakan salah satu jenis anggrek asli Indonesia dengan nilai komersial yang tinggi, tetapi anggrek ini cukup sulit dibudidayakan karena bijinya bersifat mikroskopis dan tidak memiliki endosperm sehingga perlu dibudidayakan secara in vitro. Budidaya anggrek secara in vitro memerlukan medium pendukung yang sesuai. Penelitian ini bertujuan untuk mengetahui pengaruh air kelapa (Cocos nucifera L) menggunakan medium VW terhadap pertumbuhan protocorm anggrek Phalaenopsis sp secara in vitro. Telah dilakukan penelitian di Laboratorium Kultur Jaringan DD Orchid Nursery Dadaprejo Junrejo Kabupaten Batu Jawa Timur, dimulai dari bulan November sampai dengan bulan Januari 2020. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) untuk membandingkan beberapa konsentrasi air kelapa (Cocos nucifera L) yang berbeda sebagai perlakuan yaitu, 0 %, 15%, 30%, dan 60 %. Masing-masing konsentrasi dilakukan 5 kali pengulangan dan setiap pengulangan terdiri dari 5 botol protocorm Phalaenopsis sp dan dikultur selama 4 minggu. Hasil penelitian menunjukkan bahwa jumlah protokrom dan tunas terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah 64 protocorm dan 14 tunas.Kata kunci : Air kelapa (Cocos nucifera L), Phalaenopsis sp., in vitro, pertumbuhan.
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Publisher |
FMIPA UNISMA
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Contributor |
—
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Date |
2020-08-12
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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Format |
application/pdf
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Identifier |
http://riset.unisma.ac.id/index.php/mipa/article/view/7208
10.33474/j.sa.v3i1.7208 |
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Source |
Jurnal SAINS ALAMI (Known Nature); Vol 3, No 1 (2020)
2657-1692 |
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Language |
eng
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Relation |
http://riset.unisma.ac.id/index.php/mipa/article/view/7208/6639
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Rights |
Copyright (c) 2020 Jurnal SAINS ALAMI (Known Nature)
http://creativecommons.org/licenses/by/4.0 |
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